సియస్ఐఆర్ - కణ మరియు అణు జీవవిజ్ఞాన కేంద్రం
ది ఇన్నోవేషన్ ఇంజిన్ ఆఫ్ ఇండియా
మంజుల రెడ్డి
జె సి బోస్ ఫెలోస్
బాక్టీరియల్ సెల్ వాల్ సంశ్లేషణ మరియు దాని నియంత్రణ
2514
manjula[at]ccmb[dot]res[dot]in
పరిశోధన ఆసక్తులు
మా ప్రయోగశాలలో పరిశోధన కణ చక్రంలో బ్యాక్టీరియా తమ కణ గోడలను ఎలా పొడిగిస్తుంది, విభజించి, విభజిస్తుంది, రెండు సమాన కుమార్తె కణాలను విజయవంతంగా ఉత్పత్తి చేస్తుందో అర్థం చేసుకోవడంపై దృష్టి సారించింది. ఈ ప్రశ్నలను పరిష్కరించడానికి మేము జన్యుశాస్త్రం, జీవరసాయన శాస్త్రం, కణ జీవశాస్త్రం మరియు జన్యుశాస్త్రంతో సహా బహుళ-విభాగ విధానాన్ని తీసుకుంటాము. మేము ప్రసిద్ధ గ్రామ్-నెగటివ్ రాడ్-ఆకారపు బాక్టీరియం ఎస్చెరిచియా కోలిని మా ప్రాథమిక నమూనా వ్యవస్థగా ఉపయోగిస్తాము. ఈ రంగంలో మా అత్యంత ముఖ్యమైన సహకారం బాక్టీరియల్ కణ గోడ యొక్క ప్రత్యేక భాగం అయిన పెప్టిడోగ్లైకాన్ పెరుగుదల మరియు సంశ్లేషణలో రేటు-పరిమితం చేసే క్లీవేజ్ దశను గుర్తించడం. మా పని బాక్టీరియల్ ఫిజియాలజీ యొక్క ప్రాథమిక అంశాలను బాగా అర్థం చేసుకోవడానికి దోహదపడుతుందని, తద్వారా ప్రత్యామ్నాయ యాంటీమైక్రోబయల్ థెరప్యూటిక్స్ అభివృద్ధికి కొత్త వ్యూహాలను అందిస్తుందని మేము ఆశిస్తున్నాము.
ఎంచుకున్న ప్రచురణలు
రాజ్గురు V, ఛటర్జీ S, గార్డే S, మరియు రెడ్డి M 2024. క్రాస్లింక్ క్లీవింగ్ ఎంజైమ్లు: బ్యాక్టీరియా కణ గోడను పునర్నిర్మించే స్మార్ట్ ఆటోలిసిన్లు. మైక్రోబయాలజీలో ట్రెండ్లు doi: 10.1016/j.tim.2023.11.004
Som, N మరియు రెడ్డి, M. 2023. సెల్ గోడ హైడ్రోలేస్ ద్వారా ఫాస్ఫోలిపిడ్ సంశ్లేషణ మరియు పెప్టిడోగ్లైకాన్ విస్తరణ మధ్య క్రాస్-టాక్. Proc. Natl. Acad. Sci USA.120(24), e2300784120.
బహదూర్ R, Chodisetti PK మరియు రెడ్డి M. 2021. L,D-ట్రాన్స్పెప్టిడేస్ల పారలాగ్ ద్వారా బాక్టీరియల్ పెప్టిడోగ్లైకాన్ నుండి బ్రాన్స్ లిపోప్రొటీన్ Lpp యొక్క చీలిక, LdtF. Proc. Natl. Acad. Sci USA 118: 19(2).
చోడిసెట్టి పికె మరియు రెడ్డి ఎం. 2019. అసాధారణ క్రాస్-లింక్ స్పెసిఫిసిటీ యొక్క పెప్టిడోగ్లైకాన్ హైడ్రోలేస్ బాక్టీరియల్ సెల్ వాల్ సంశ్లేషణకు దోహదం చేస్తుంది. ప్రోక్. నేషనల్. అకాడ్. సైన్స్ యుఎస్ఎ 116: 7825–7830.
విద్య మరియు అనుభవం
P.G:
బయోకెమిస్ట్రీ; హైదరాబాద్ విశ్వవిద్యాలయం; 1985
Ph.D:
వివిధ దశల పెరుగుదలలో ఎస్చెరిచియా కోలిలో ఉత్పరివర్తన విధానాల అధ్యయనాలు: లాక్జెడ్ జన్యువులోని ఉత్పరివర్తనాల తిరోగమన విశ్లేషణ; జవహర్లాల్ నెహ్రూ విశ్వవిద్యాలయం (JNU-CCMB), న్యూఢిల్లీ; 2001
టీమ్ సభ్యులు
Manjula Reddy
J C Bose Fellow
G Bhargavi Krishna Sree
Senior Research Fellow
Shambhavi Garde
Senior Research Fellow
Suraj Kumar Meher
Junior Research Fellow
Soma Banerjee
Junior Research Fellow
Krishna Chaitanya N
Junior Research Fellow
Stuti Chatterjee
Junior Research Fellow
Vishnu Vachana Murapaka
Junior Research Fellow
Vaidehi Rajguru
Senior Project Associate
Debnita Mongal
Junior Research Fellow (DBT)
Syeda Meher Fatima
Project Assistant
Harikrishnan S
Project Associate - I
ప్రకాశనాలు
శీర్షిక
పత్రిక
సంవత్సరం
Glycan strand cleavage by a lytic transglycosylase, MltD contributes to the expansion of peptidoglycan in Escherichia coli.
PLoS genetics
2024
Crosslink cleaving enzymes: the smart autolysins that remodel the bacterial cell wall.
Trends in Microbiology
2023
Cross-talk between phospholipid synthesis and peptidoglycan expansion by a cell wall hydrolase
Proceedings of the National Academy of Sciences
2023
Peptidoglycan Remodeling by an L,D-Transpeptidase, LdtD during Cold Shock in Escherichia coli
Journal of Bacteriology
2022
Modulation of RecFORQ- and RecA-mediated homologous recombination in Escherichia coli by isoforms of translation initiation factor IF2
Journal of Bacteriology
2022
Structural basis for the peptidoglycan editing activity of YfiH
mBio
2022
A LytM-factor, ActS functions in two distinctive peptidoglycan hydrolytic pathways in Escherichia coli
Frontiers in Microbiology
2022
Cleavage of Braun`s lipoprotein Lpp from the bacterial peptidoglycan by a paralog of L,D-transpeptidases, LdtF
PNAS
2021
Peptidoglycan: structure, synthesis and regulation
EcoSal Plus
2021
Structural basis for the differential regulatory roles of the PDZ domain in C-terminal processing proteases.
mBio
2019
Peptidoglycan hydrolase of an unusual cross-link specificity contributes to bacterial cell wall synthesis.
Proc Natl Acad Sci. USA
2019
A synergistic role for two predicted inner membrane proteins of Escherichia coli in cell envelope integrity.
Molecular Microbiology
2019
Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc.
Nature Communications
2017
Determination of Keto-deoxy-d-manno-8-octanoic acid (KDO) from Lipopolysaccharide of Escherichia coli.
Bio-protocol
2015
Regulated proteolysis of a crosslink-specific peptidoglycan hydrolase contributes to bacterial morphogenesis.
Proc. Natl. Acad. Sci USA
2015
yciM is an essential gene required for regulation of lipopolysaccharide synthesis in Escherichia coli.
Molecular Microbiology
2014
Three redundant murein endopeptidases catalyse an essential cleavage step in peptidoglycan synthesis of Escherichia coli K12.
Molecular Microbiology
2012
SufI (FtsP) is a cell division protein required for the growth of Escherichia coli during oxidative stress.
Journal of Bacteriology
2007
Role of FtsEX in cell division of Escherichia coli: viability of ftsEX mutants is dependent on functional SufI or high osmotic strength.
Journal of Bacteriology
2007
Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hotspots.
Genes & Development
2007
A positive selection system for identification of recombinants using alpha-complementation plasmids.
BioTechniques
2004
Distinct signatures for mutator sensitivity of lacZ reversions, and for spectrum of lacI/lacO forward mutations on the chromosome of nondividing Escherichia coli.
Genetics
2004
IS186 insertion at a hot spot in the lon promoter as a basis for Lon protease deficiency of Escherichia coli B: Identification of a consensus target sequence for IS186 transposition.
Journal of Bacteriology
2001
lon incompatibility associated with mutations causing SOS induction: Null uvrD alleles induce an SOS response in Escherichia coli.
Journal of Bacteriology
2000
Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli.
Journal of Bacteriology
2000
A genetic strategy to demonstrate the occurrence of spontaneous mutations in nondividing cells within colonies of Escherichia coli.
Genetics
1997
Identification and characterization of ssb and uup mutants with increased frequency of precise excision of transposon Tn10 derivatives: nucleotide sequence of uup in Escherichia coli.
Journal of Bacteriology
1997
A conserved editing mechanism for the fidelity of bacterial cell wall biosynthesis
Proceedings of the National Academy of Sciences U S A
2025
Identification of SanA as a novel regulator of peptidoglycan biogenesis in Escherichia coli
PLoS Genetics
2025
Identification of YfiH (PgeF) as a factor contributing to the maintenance of bacterial peptidoglycan composition.
Molecular Microbiology
2019
Identification of YfiH (PgeF) as a factor contributing to the maintenance of bacterial peptidoglycan composition.
Molecular Microbiology
2019
Identification of YfiH (PgeF) as a factor contributing to the maintenance of bacterial peptidoglycan composition.
Molecular Microbiology
2019
Identification of SanA as a novel regulator of peptidoglycan biogenesis in Escherichia coli
PLoS Genetics
2025
Identification of SanA as a novel regulator of peptidoglycan biogenesis in Escherichia coli
PLoS Genetics
2025
A conserved editing mechanism for the fidelity of bacterial cell wall biosynthesis
Proceedings of the National Academy of Sciences U S A
2025
A conserved editing mechanism for the fidelity of bacterial cell wall biosynthesis
Proceedings of the National Academy of Sciences U S A
2025
Identification and characterization of ssb and uup mutants with increased frequency of precise excision of transposon Tn10 derivatives: nucleotide sequence of uup in Escherichia coli.
Journal of Bacteriology
1997
Identification and characterization of ssb and uup mutants with increased frequency of precise excision of transposon Tn10 derivatives: nucleotide sequence of uup in Escherichia coli.
Journal of Bacteriology
1997
A genetic strategy to demonstrate the occurrence of spontaneous mutations in nondividing cells within colonies of Escherichia coli.
Genetics
1997
A genetic strategy to demonstrate the occurrence of spontaneous mutations in nondividing cells within colonies of Escherichia coli.
Genetics
1997
Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli.
Journal of Bacteriology
2000
Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli.
Journal of Bacteriology
2000
lon incompatibility associated with mutations causing SOS induction: Null uvrD alleles induce an SOS response in Escherichia coli.
Journal of Bacteriology
2000
lon incompatibility associated with mutations causing SOS induction: Null uvrD alleles induce an SOS response in Escherichia coli.
Journal of Bacteriology
2000
IS186 insertion at a hot spot in the lon promoter as a basis for Lon protease deficiency of Escherichia coli B: Identification of a consensus target sequence for IS186 transposition.
Journal of Bacteriology
2001
IS186 insertion at a hot spot in the lon promoter as a basis for Lon protease deficiency of Escherichia coli B: Identification of a consensus target sequence for IS186 transposition.
Journal of Bacteriology
2001
Distinct signatures for mutator sensitivity of lacZ reversions, and for spectrum of lacI/lacO forward mutations on the chromosome of nondividing Escherichia coli.
Genetics
2004
Distinct signatures for mutator sensitivity of lacZ reversions, and for spectrum of lacI/lacO forward mutations on the chromosome of nondividing Escherichia coli.
Genetics
2004
A positive selection system for identification of recombinants using alpha-complementation plasmids.
BioTechniques
2004
A positive selection system for identification of recombinants using alpha-complementation plasmids.
BioTechniques
2004
Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hotspots.
Genes & Development
2007
Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hotspots.
Genes & Development
2007
Role of FtsEX in cell division of Escherichia coli: viability of ftsEX mutants is dependent on functional SufI or high osmotic strength.
Journal of Bacteriology
2007
Role of FtsEX in cell division of Escherichia coli: viability of ftsEX mutants is dependent on functional SufI or high osmotic strength.
Journal of Bacteriology
2007
SufI (FtsP) is a cell division protein required for the growth of Escherichia coli during oxidative stress.
Journal of Bacteriology
2007
SufI (FtsP) is a cell division protein required for the growth of Escherichia coli during oxidative stress.
Journal of Bacteriology
2007
Three redundant murein endopeptidases catalyse an essential cleavage step in peptidoglycan synthesis of Escherichia coli K12.
Molecular Microbiology
2012
Three redundant murein endopeptidases catalyse an essential cleavage step in peptidoglycan synthesis of Escherichia coli K12.
Molecular Microbiology
2012
yciM is an essential gene required for regulation of lipopolysaccharide synthesis in Escherichia coli.
Molecular Microbiology
2014
yciM is an essential gene required for regulation of lipopolysaccharide synthesis in Escherichia coli.
Molecular Microbiology
2014
Regulated proteolysis of a crosslink-specific peptidoglycan hydrolase contributes to bacterial morphogenesis.
Proc. Natl. Acad. Sci USA
2015
Regulated proteolysis of a crosslink-specific peptidoglycan hydrolase contributes to bacterial morphogenesis.
Proc. Natl. Acad. Sci USA
2015
Determination of Keto-deoxy-d-manno-8-octanoic acid (KDO) from Lipopolysaccharide of Escherichia coli.
Bio-protocol
2015
Determination of Keto-deoxy-d-manno-8-octanoic acid (KDO) from Lipopolysaccharide of Escherichia coli.
Bio-protocol
2015
Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc.
Nature Communications
2017
Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc.
Nature Communications
2017
A synergistic role for two predicted inner membrane proteins of Escherichia coli in cell envelope integrity.
Molecular Microbiology
2019
A synergistic role for two predicted inner membrane proteins of Escherichia coli in cell envelope integrity.
Molecular Microbiology
2019
Peptidoglycan hydrolase of an unusual cross-link specificity contributes to bacterial cell wall synthesis.
Proc Natl Acad Sci. USA
2019
Peptidoglycan hydrolase of an unusual cross-link specificity contributes to bacterial cell wall synthesis.
Proc Natl Acad Sci. USA
2019
Structural basis for the differential regulatory roles of the PDZ domain in C-terminal processing proteases.
mBio
2019
Structural basis for the differential regulatory roles of the PDZ domain in C-terminal processing proteases.
mBio
2019
Peptidoglycan: structure, synthesis and regulation
EcoSal Plus
2021
Peptidoglycan: structure, synthesis and regulation
EcoSal Plus
2021
Cleavage of Braun`s lipoprotein Lpp from the bacterial peptidoglycan by a paralog of L,D-transpeptidases, LdtF
PNAS
2021
Cleavage of Braun`s lipoprotein Lpp from the bacterial peptidoglycan by a paralog of L,D-transpeptidases, LdtF
PNAS
2021
A LytM-factor, ActS functions in two distinctive peptidoglycan hydrolytic pathways in Escherichia coli
Frontiers in Microbiology
2022
A LytM-factor, ActS functions in two distinctive peptidoglycan hydrolytic pathways in Escherichia coli
Frontiers in Microbiology
2022
Structural basis for the peptidoglycan editing activity of YfiH
mBio
2022
Structural basis for the peptidoglycan editing activity of YfiH
mBio
2022
Modulation of RecFORQ- and RecA-mediated homologous recombination in Escherichia coli by isoforms of translation initiation factor IF2
Journal of Bacteriology
2022
Modulation of RecFORQ- and RecA-mediated homologous recombination in Escherichia coli by isoforms of translation initiation factor IF2
Journal of Bacteriology
2022
Peptidoglycan Remodeling by an L,D-Transpeptidase, LdtD during Cold Shock in Escherichia coli
Journal of Bacteriology
2022
Peptidoglycan Remodeling by an L,D-Transpeptidase, LdtD during Cold Shock in Escherichia coli
Journal of Bacteriology
2022
Cross-talk between phospholipid synthesis and peptidoglycan expansion by a cell wall hydrolase
Proceedings of the National Academy of Sciences
2023
Cross-talk between phospholipid synthesis and peptidoglycan expansion by a cell wall hydrolase
Proceedings of the National Academy of Sciences
2023
Crosslink cleaving enzymes: the smart autolysins that remodel the bacterial cell wall.
Trends in Microbiology
2023
Crosslink cleaving enzymes: the smart autolysins that remodel the bacterial cell wall.
Trends in Microbiology
2023
Glycan strand cleavage by a lytic transglycosylase, MltD contributes to the expansion of peptidoglycan in Escherichia coli.
PLoS genetics
2024
Glycan strand cleavage by a lytic transglycosylase, MltD contributes to the expansion of peptidoglycan in Escherichia coli.
PLoS genetics
2024