Training Program for In-House Users: Handling Sophisticated Imaging Systems

1. Access Control & Training (In-house users)

• Lecture (2 hours):
• Basics of microscopy
• Fluorescence microscopy
• Digital imaging techniques

2. System Demonstrations

• Basics of Microscopy and Fluorescence Microscopy (2.5 hours):
• Transmitted light microscopy techniques: Bright field, DIC, Phase contrast,
  Darkfield
• Kohler illumination
• Fluorescence microscopy
• Hands-on: Operate Leica DM6 fluorescence microscope and complete
  assignment
•  Confocal Microscopy (2 hours):
• Demonstration of Leica SP8 / Zeiss LSM 880 confocal microscope
• Overview of confocal imaging principles and setup

3. Examination

• Online MCQ Test (45 minutes):
• Complete multiple-choice questionnaire on microscopy concepts and
  techniques
• Instant results upon submission

4. Confocal Hands-on Exercise (2-3 hours)

• Practical Session:
• Users complete assignment using sub-resolution beads on the Leica SP8
  Confocal/ LSM 880 confocal microscope
• Independent operation to apply learned concepts

5. Submission of Assignments

• Submit assignment results after completing the hands-on exercise

6. Facility Access

• Facility Access: Gain access to the imaging facility after assessment by the
  facility in charge

This structured, point-wise program ensures that users receive comprehensive training,
theoretical knowledge, and hands-on practice to confidently operate imaging systems.

Training Programs/SDP

The Advanced Microscopy and Imaging Facility (AMIF) conducts an annual microscopy
course, offering hands-on training in cutting-edge imaging techniques. This intensive
program covers confocal, light-sheet, and super-resolution microscopy, with expert-led
sessions on sample preparation, image acquisition, and analysis. Designed for researchers
and students, the course provides a unique opportunity to explore the latest advancements
in microscopy and enhance imaging skills for biological research.

Resources

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Publications